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eco ri  (New England Biolabs)


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  • 99

    Structured Review

    New England Biolabs eco ri
    Eco Ri, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 15403 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eco+ri/EcoRI/pmc13124767-47-11-16
    Average 99 stars, based on 15403 article reviews
    eco ri - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Gut microbiota within-host evolution enforces colonization resistance against enteric infection
    Article Snippet: .. Briefly, homologous regions upstream and downstream of deglycase genes were amplified from E. faecalis SW160 genomic DNA via PCR and assembled with an erythromycin resistance gene( ) and Eco RI- and Bam HI-restricted plasmid pLT06 using the NEBuilder HiFi DNA Assembly Master Mix. .. Enzymes and assembly reagents were obtained from New England Biolabs (U.S.A.) and used according to the manufacturer’s instructions.

    Article Title: A versatile plasmid platform for auxotrophic complementation in attenuated Mycobacterium bovis BCG
    Article Snippet: The expression cassette containing the leuD gene was amplified from pUP410, and the egfp sequence was amplified from pRSETEmGFP (Invitrogen), using primers described previously [ ]. .. The pAN- leuD expression cassette was amplified by PCR, digested with Eco RI and Spe I (New England Biolabs), and ligated upstream of the promoter region in pUP500 vectors previously digested with Eco RI and Xba I (New England Biolabs), using T4 DNA ligase (Invitrogen). ..

    Polymerase Chain Reaction:

    Article Title: Gut microbiota within-host evolution enforces colonization resistance against enteric infection
    Article Snippet: .. Briefly, homologous regions upstream and downstream of deglycase genes were amplified from E. faecalis SW160 genomic DNA via PCR and assembled with an erythromycin resistance gene( ) and Eco RI- and Bam HI-restricted plasmid pLT06 using the NEBuilder HiFi DNA Assembly Master Mix. .. Enzymes and assembly reagents were obtained from New England Biolabs (U.S.A.) and used according to the manufacturer’s instructions.

    Article Title: Chromosome-level genome assembly of triploid Cyperus rotundus uncovers allelic expression patterns and herbicide resistance mechanisms
    Article Snippet: .. Purified PCR products were cloned into pYES2 vectors linearized with Kpn I/ Eco RI (NEB) digestion using In-Fusion assembly (TaKaRa, Japan). ..

    Article Title: A versatile plasmid platform for auxotrophic complementation in attenuated Mycobacterium bovis BCG
    Article Snippet: The expression cassette containing the leuD gene was amplified from pUP410, and the egfp sequence was amplified from pRSETEmGFP (Invitrogen), using primers described previously [ ]. .. The pAN- leuD expression cassette was amplified by PCR, digested with Eco RI and Spe I (New England Biolabs), and ligated upstream of the promoter region in pUP500 vectors previously digested with Eco RI and Xba I (New England Biolabs), using T4 DNA ligase (Invitrogen). ..

    Plasmid Preparation:

    Article Title: Gut microbiota within-host evolution enforces colonization resistance against enteric infection
    Article Snippet: .. Briefly, homologous regions upstream and downstream of deglycase genes were amplified from E. faecalis SW160 genomic DNA via PCR and assembled with an erythromycin resistance gene( ) and Eco RI- and Bam HI-restricted plasmid pLT06 using the NEBuilder HiFi DNA Assembly Master Mix. .. Enzymes and assembly reagents were obtained from New England Biolabs (U.S.A.) and used according to the manufacturer’s instructions.

    Article Title: Both genome instability and replicative senescence stem from the shortest telomere in telomerase-negative cells.
    Article Snippet: Plates containing 500-10,000 colonies were scraped and plasmids were extracted in batch with the NucleoSpin Plasmid kit (Macherey Nagel #740588.50). .. ~1 μg of plasmid DNA was linearized with Eco RI (NEB #R3101) and libraries of 12 samples were multiplexed using the Ligation sequencing gDNA - Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies SQK-NBD114.24) and run on a MinION flowcell (v.10.4.1). ..

    Ligation:

    Article Title: Both genome instability and replicative senescence stem from the shortest telomere in telomerase-negative cells.
    Article Snippet: Plates containing 500-10,000 colonies were scraped and plasmids were extracted in batch with the NucleoSpin Plasmid kit (Macherey Nagel #740588.50). .. ~1 μg of plasmid DNA was linearized with Eco RI (NEB #R3101) and libraries of 12 samples were multiplexed using the Ligation sequencing gDNA - Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies SQK-NBD114.24) and run on a MinION flowcell (v.10.4.1). ..

    Sequencing:

    Article Title: Both genome instability and replicative senescence stem from the shortest telomere in telomerase-negative cells.
    Article Snippet: Plates containing 500-10,000 colonies were scraped and plasmids were extracted in batch with the NucleoSpin Plasmid kit (Macherey Nagel #740588.50). .. ~1 μg of plasmid DNA was linearized with Eco RI (NEB #R3101) and libraries of 12 samples were multiplexed using the Ligation sequencing gDNA - Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies SQK-NBD114.24) and run on a MinION flowcell (v.10.4.1). ..

    Purification:

    Article Title: Chromosome-level genome assembly of triploid Cyperus rotundus uncovers allelic expression patterns and herbicide resistance mechanisms
    Article Snippet: .. Purified PCR products were cloned into pYES2 vectors linearized with Kpn I/ Eco RI (NEB) digestion using In-Fusion assembly (TaKaRa, Japan). ..

    Clone Assay:

    Article Title: Chromosome-level genome assembly of triploid Cyperus rotundus uncovers allelic expression patterns and herbicide resistance mechanisms
    Article Snippet: .. Purified PCR products were cloned into pYES2 vectors linearized with Kpn I/ Eco RI (NEB) digestion using In-Fusion assembly (TaKaRa, Japan). ..

    Article Title: Compound Inflorescence (S) represses fruit growth and seed development in tomato
    Article Snippet: .. The oligos for the two guide RNAs were cloned into the psgR-Cas9-At vector, and then the expression cassette released using Eco RI and Hind III (New England BioLabs, USA) was cloned into pCAMBIA1300. ..

    Expressing:

    Article Title: A versatile plasmid platform for auxotrophic complementation in attenuated Mycobacterium bovis BCG
    Article Snippet: The expression cassette containing the leuD gene was amplified from pUP410, and the egfp sequence was amplified from pRSETEmGFP (Invitrogen), using primers described previously [ ]. .. The pAN- leuD expression cassette was amplified by PCR, digested with Eco RI and Spe I (New England Biolabs), and ligated upstream of the promoter region in pUP500 vectors previously digested with Eco RI and Xba I (New England Biolabs), using T4 DNA ligase (Invitrogen). ..

    Article Title: Compound Inflorescence (S) represses fruit growth and seed development in tomato
    Article Snippet: .. The oligos for the two guide RNAs were cloned into the psgR-Cas9-At vector, and then the expression cassette released using Eco RI and Hind III (New England BioLabs, USA) was cloned into pCAMBIA1300. ..



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